FAM tetrazine, 5-isomer

Cat. # Quantity Price Lead time
141E0 1 mg $110 in stock
241E0 5 mg $210 in stock
441E0 25 mg $410 in stock
541E0 50 mg $695 in stock
641E0 100 mg $1190 in stock
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Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.

Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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General properties

Appearance: yellow solid
Mass spec M+ increment: 529.12
Molecular weight: 559.53
CAS number: 1619222-85-3
Molecular formula: C31H21N5O6
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17
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