FAM tetrazine, 5-isomer

FAM (Fluorescein) Tetrazines Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
141E0	1 mg	$110	in stock
241E0	5 mg	$210	in stock
441E0	25 mg	$410	in stock
541E0	50 mg	$695	in stock
641E0	100 mg	$1190	in stock

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Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.

Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.

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FAM absorbance and emission spectra

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General properties

Appearance:	yellow solid
Mass spec M+ increment:	529.12
Molecular weight:	559.53
CAS number:	1619222-85-3
Molecular formula:	C₃₁H₂₁N₅O₆
Solubility:	good in DMF, DMSO
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	492
ε, L⋅mol⁻¹⋅cm⁻¹:	74000
Emission maximum, nm:	517
Fluorescence quantum yield:	0.93
CF₂₆₀:	0.22
CF₂₈₀:	0.17