FAM tetrazine, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151E0 | 1 mg | $110 | in stock | |
| 251E0 | 5 mg | $210 | in stock | |
| 451E0 | 25 mg |
$410
|
in stock | |
| 551E0 | 50 mg |
$695
|
in stock | |
| 651E0 | 100 mg |
$1190
|
in stock |
Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of the visible spectrum. This fluorescein derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick and specific. This reagent is a pure 6-isomer of FAM.
Absorption and emission spectra of FAM
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Trans-cyclooctene (TCO) amidite is used to introduce a TCO moiety into substrates containing primary or secondary hydroxyl group
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FAM tetrazine, 5-isomer
FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 531.1 |
| Molecular weight: | 559.53 |
| Molecular formula: | C31H21N5O6 |
| Solubility: | good in DMF, DMSO, poor in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
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