FAM tetrazine, 6-isomer

FAM (Fluorescein) Tetrazines Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
151E0	1 mg	$110	in stock
251E0	5 mg	$210	in stock
451E0	25 mg	$410	in stock
551E0	50 mg	$695	in stock
651E0	100 mg	$1190	in stock

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Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of the visible spectrum. This fluorescein derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick and specific. This reagent is a pure 6-isomer of FAM.

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Absorption and emission spectra of FAM

General properties

Appearance:	orange solid
Mass spec M+ increment:	531.1
Molecular weight:	559.53
Molecular formula:	C₃₁H₂₁N₅O₆
Solubility:	good in DMF, DMSO, poor in water
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	492
ε, L⋅mol⁻¹⋅cm⁻¹:	74000
Emission maximum, nm:	517
Fluorescence quantum yield:	0.93
CF₂₆₀:	0.22
CF₂₈₀:	0.17

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.