FAM isothiocyanate (FITC), 5- and 6-isomers
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3524-100mg | 100 mg | $45 | in stock | |
| 3524-500mg | 500 mg | $249 | in stock |
FAM (5,6)-isothiocyanate or (5,6)-FITC is an isothiocyanate derivative of fluorescein that reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules. It is often used to label antibodies and other probes for use in various fluorescence-based techniques such as microscopy, flow cytometry, and immunofluorescence assays like immunochemistry, Western blotting, and ELISA.
FAM absorbance and emission spectra
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A Cyanine3B derivative with unactivated free carboxy group for use as a control, calibration, and conjugation after pre-activation.General properties
| Appearance: | orange powder |
| Molecular weight: | 389.38 |
| CAS number: | 27072-45-3 |
| Molecular formula: | C21H11NO5S |
| Solubility: | good in DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| Emission maximum, nm: | 517 |
Product citations
- Lysakova, E.; Shumeev, A.; Laktyushkin, V.; Chuvpilo, S.; Rybtsov, S. Assay Development for Phagocytosis Activity Evaluation. Biol. Life Sci. Forum, 2024, 31(1), 15. doi: 10.3390/ecm2023-16444
- Zhang, Y.; Wang, H.; Zheng, Y.; Wu, Z.; Liu, J.; Cheng, F.; Wang, K. Degradation of Angelica sinensis polysaccharide: Structures and protective activities against ethanol-induced acute liver injury. Carbohydrate polymers, 2024, 328, 121745. doi: 10.1016/j.carbpol.2023.121745
- Wu, F.; Ren, Y.; Lv, W.; Liu, X.; Wang, X.; Wang, C.; Cao, Z.; Liu, J.; Wei, J.; Pang, Y. Generating dual structurally and functionally skin-mimicking hydrogels by crosslinking cell-membrane compartments. Nature Communications, 2024, 15(1), 802. doi: 10.1038/s41467-024-45006-7
- Luo, T.; Jiang, M.; Cheng, Z.; Lin, Y.; Chen, Y.; Zhang, Z.; Zhou, J.; Zhou, W.; Yu, X.-F.; Li, S.; Geng, S.; Yang, H. Biodegradable FePS3 nanoplatform for efficient treatment of osteosarcoma by combination of gene and NIR-II photothermal therapy. Journal of Nanobiotechnology, 2023, 21, 224. doi: 10.1186/s12951-023-01961-9
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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