LumiMount DAPI Fluorescence Mounting Medium
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13144 | 2 mL | – | in stock | |
| 53144 | 15 mL | $89 | in stock |
LumiMount DAPI is an aqueous-based mounting medium with DAPI (4′,6-diamidino-2-phenylindole) for coverslipping and imaging fluorescently labeled cell or tissue samples.
DAPI is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA and is used as a chromosome staining and nuclear counterstain. When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 358 nm and emission maximum at 461 nm.
LumiMount DAPI is compatible with most fluorescent markers used in microscopy applications and can reduce fluorochrome quenching during imaging due to anti-fade agent content. The refractive index of liquid mounting medium is 1.38, but it can increase to 1.46 as the water evaporates at the edges of the coverslip.
LumiMount DAPI provides a semi-permanent seal for prolonged slide storage at 2°C to 8°C. Since LumiMount DAPI is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened. For better sealing of slides, it is recommended to seal the edges of the coverslip with waterproof nail polish.
Absorption and emission spectra of DAPI
Recommended protocol
Calculator
Customers also purchased with this product
AF 647 azide
Bright and photostable azide AF 647 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.DiI, lipophilic tracer
Orange-red fluorescent lipophilic dye for membrane labeling and cell tracing.LumiTracker® Mito Green FM
Сell-permeant cationic green-fluorescent dye for staining active mitochondria in living cells in a potential-dependent manner.General properties
| Appearance: | colorless liquid |
| Solubility: | good in water |
| Quality control: | refractive index, viscosity |
| Storage conditions: | Storage: 24 months after receival at 2-8°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid exposure to light. |
| MSDS: | Download |
| Product specifications |
Your cart is empty
$ 
