C6 Spacer phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 24160 | 250 mg |
$269
$169
|
in stock | |
| 44160 | 1 g |
$837
$642
|
in stock | |
| 74160 | 10 g | please inquire | 21 days |
C6 spacer phosphoramidite serves as a crucial tool in nucleic acid chemistry, enabling researchers to design more effective oligonucleotides by incorporating hydrophobic spacers that enhance performance in various biochemical applications. Its ability to minimize unwanted interactions while providing structural flexibility makes it an invaluable reagent in modern molecular biology techniques. Multiple spacers can be added to achieve precise control over the length of the spacer arms, which is essential in studies involving hairpin loops and duplex formations.
The C6 spacer is hydrophobic, making it suitable for applications where water solubility is not critical. It effectively distances fluorescent dyes or other modifications from the oligonucleotide sequence, reducing quenching effects. For example, G-rich sequences can quench fluorescein; adding a spacer helps mitigate this issue.
This spacer can be utilized in various contexts, including PCR amplification and hybridization assays. It allows for flexibility in the design of probes and primers, which can be crucial for specific applications like Scorpion Primers and SMART detection assays.
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| Appearance: | colorless oil |
| Molecular weight: | 620.77 |
| Molecular formula: | C36H49N2O5P |
| Solubility: | DCM, acetonitrile, DMF, DMSO. Sensitive to moisture in solvents |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | anhydrous acetonitrile |
| Coupling conditions: | standard conditions identical to normal nucleobases. Tolerates oxidation step conditions. |
| Cleavage conditions: | in concentrated ammonium hydroxide for 5 hours at 60 °C (or 1 hour for fast-deprotecting amidites). AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65 °C. |
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