dsGreen for Real-Time PCR, 100×
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A1010 | 50 uL | – | in stock | |
11010 | 100 uL | $39 | in stock | |
41010 | 1 mL | $235 | in stock | |
71010 | 5 mL | $510 | in stock | |
91010 | 50 mL | $2670 | in stock |
dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Excitation and emission spectra of dsDNA complex with dsGreen
Recommended protocol
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TAMRA azide, 5-isomer
Azide derivative of TAMRA fluorescent dye for сlick chemistry.Ascorbic acid
Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.BDP TMR NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.General properties
Appearance: | light orange solution |
Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 490 |
ε, L⋅mol−1⋅cm−1: | 73000 |
Emission maximum, nm: | 524 |
Fluorescence quantum yield: | 0,8 |
Product citations
- Bykonia, E.N.; Kleymenov, D.A.; Gushchin, V.A.; Sinyavin, A.E.; Mazunina, E.P.; Kuznetsova, N.A.; Kozlova, S.R.; Zolotar, A.N.; Shidlovskaya, E.V.; Usachev, E.V.; Pochtovyi, A.A.; Kustova, D.D.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Major role of S-glycoprotein in providing immunogenicity and protective immunity in mRNA lipid nanoparticle vaccines based on SARS-CoV-2 structural proteins. bioRxiv, 2024, preprint. doi: 10.1101/2023.12.30.573713
- Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
- Sakhabutdinova, A. R.; Chemeris, A. V.; Garafutdinov, R. R. Detection of Specific RNA Targets by Multimerization. Biochemistry (Moscow), 2023, 88(5), 679–686. doi: 10.1134/S0006297923050103
- Hildebrandt, R.P.; Moss, K.R.; Janusz-Kaminska, A.; Knudson, L.A.; Denes, L.T.; Saxena, T.; Boggupalli, D.P.; Li, Z.; Lin, K.; Bassell, G.J.; Wang, E.T. Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes. Nature Communications, 2023, 14, 3427. doi: 10.1038/s41467-023-38923-6
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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