TAMRA CPG 500, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2792-100mg | 100 mg | $95 | in stock | |
| 2792-500mg | 500 mg | $295 | in stock | |
| 2792-1g | 1 g |
$469
|
in stock | |
| 2792-5g | 5 g | please inquire | in stock |
Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.
TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.
The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: standard time depending on the first monomer.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
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VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.Copper(II)-TВTA catalytic buffer, 1.5x
Ready-to-use catalytic buffer containing сopper(II) and TBTA ligand. It is suitable for click chemistry modification of nucleic acids and small molecules.General properties
| Appearance: | deep pink beads |
| Quality control: | NMR 1H and loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−70 |
Product citations
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
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