TAMRA phosphoramidite, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2497-100mg | 100 mg | please inquire | in stock (3 pcs) | |
| 2497-250mg | 250 mg | please inquire | in stock (2 pcs) | |
| 2497-1g | 1 g | please inquire | in stock (2 pcs) | |
| 2497-5g | 5 g | please inquire | in stock (2 pcs) |
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.
TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).
TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: 7.5 min.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.
Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
Absorption and emission spectra of 5-TAMRA
Customers also purchased with this product
AF 532 carboxylic acid
Non-reactive form of AF 532 fluorescent dye with a free carboxyl group.JOE azide, 5-isomer
JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for сlick chemistryCyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for use in oligonucleotide synthesis.
Add this product to your cart andget free express delivery
General properties
| Appearance: | purple solid |
| Mass spec M+ increment: | 589.60 |
| Molecular weight: | 727.83 |
| Molecular formula: | C40H50N5O6P |
| Solubility: | good in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, HPLC-MS (95%), coupling test |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Diluent: | acetonitrile |
Your cart is empty
$ 
