TET carboxylic acid, 6-isomer

Cat. # Quantity Price Lead time
28290 5 mg $125 12 days
48290 25 mg $225 7 days
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TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate with absorption maximum at 519 nm and emission maximum at 535 nm. TET is spectrally similar to R6G, JOE, and VIC, widely used for labeling PCR probes.

TET carboxylic acid is a non-reactive form of TET dye that can be used as a reference standard in experiments involving TET dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.

Absorption and emission spectra of TET, 6-isomer

Absorption and emission spectra of TET, 6-isomer

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ROX carboxylic acid, 6-isomer

Derivative of ROX (Rhodamine X, Rhodamine 101) fluorophore with non-activated free carboxylic acid group. This reagent is a pure 6-isomer.

General properties

Appearance: orange powder
Molecular weight: 514.10
Molecular formula: C21H8Cl4O7
Solubility: good in DMSO, DMF, methanol, basic solutions, limited in acetonitrile
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 100000
Emission maximum, nm: 535
Fluorescence quantum yield: 0.47
CF260: 0.17
CF280: 0.09
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