TOR-G4, G-quadruplexes fluorescent probe

Cat. # Quantity Price Lead time
A5510 50 uL, 5 mM/DMSO $149 in stock
15510 500 uL, 5 mM/DMSO $390 in stock
4357-1g 1 g please inquire in stock
Found better price? Let us know and we will propose the way forward!

G-quadruplexes (G4s) are secondary structures that form in DNA and RNA through noncanonical hydrogen bonding between four guanine bases [1,2]. In the nucleus, DNA G4s have been associated with epigenetic regulation of gene expression through their interactions with regulatory proteins, such as transcription factors and chromatin modifiers [3,4]. RNA G4s have been linked to RNA splicing, transport, and translation regulation, as well as RNA-mediated stress responses in the cytoplasm [5-7].

TOR-G4 is a thiazole orange derivative, a newly synthesized G4 fluorescent probe [8]. It is a small-molecule alternative to immunochemistry with G4-specific antibodies. TOR-G4 allows the visualization of G4s based on changes to the fluorescence lifetime of a probe upon nucleic acid binding. The lifetime of TOR-G4 is highest in the presence of G4s and lower for other sequences. Within cells, TOR-G4 is primarily colocalized with RNA in the cytoplasm and nucleoli, making it the first lifetime-based probe validated for exploring the emerging roles of RNA G4s in cell functioning. TOR-G4 is suitable for imaging RNA G4s via FLIM [8].

[1] Trends in Chemistry 2, 123 (2020); [2] Nat Rev Mol Cell Biol 21, 459 (2020); [3] Nucleic Acids Res 49, 8419 (2021); [4] Trends Genet 35, 29 (2019); [5] Nucleic Acids Res 48, 12534 (2020); [6] Trends Biochem Sci 46, 270 (2021); [7] Nucleic Acids Res 49, 5426 (2021); [8] J Am Chem Soc 146, 1009 (2024).

Excitation and emission spectra of TOR-G4 (G4 DNA (c-MYC))

Excitation and emission spectra of TOR-G4 (G4 DNA (c-MYC))

Customers also purchased with this product

Azide-PEG2-amine

A diethylene glycol (PEG2) linker with azide and amino groups.

BDP® 650/665 lipid stain

Hydrophobic borondipyrromethene dye BDP 650/665 for staining of lipids and lipophilic compounds.

Laurdan (6-Dodecanoyl-2-dimethylaminonaphthalene)

Laurdan is a polarity-sensitive lipid membrane fluorescent probe. It is used for imaging lipid rafts and studying cell plasmatic membrane dynamics.

General properties

Appearance: black solid
Molecular weight: 607.56
Molecular formula: C30H30IN3OS
Solubility: good in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 483; 567 (c-MYC complex)
Emission maximum, nm: 648 (c-MYC complex)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.