TCO phosphoramidite C6

Oligonucleotide synthesis Copper-free click chemistry Phosphoramidites Cycloalkenes
Cat. # Quantity Price Lead time
4017-100mg 100 mg $480 in stock
4017-250mg 250 mg $920 in stock
4017-1g 1 g please inquire in stock
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Trans-cyclooctene (TCO) amidite is a hydroxyl reactive block used to introduce a TCO moiety into substrates containing primary or secondary hydroxyl group, and is especially useful in preparation of TCO-modified oligonucleotides.

Trans-cyclooctene readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term stability in aqueous media, which is important in low-concentration systems applications such as protein-protein conjugations, etc.

Recommendations for using the reagent:

Coupling: Standard conditions identical to normal nucleobases.

Oxidation: TCO well tolerates iodine oxidation step conditions.

Deprotection: Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.

Cleavage: AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65°C

TCO phosphoramidite C6, 4017-250mg
TCO phosphoramidite C6, 4017-250mg
TCO phosphoramidite C6, 4017-250mg
Structure of TCO phosphoramidite C6
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General properties

Appearance: colorless to yellowish oil
Molecular weight: 469.61
Molecular formula: C24H44N3O4P
Quality control: NMR 1H, NMR 31P (95+%)
Storage conditions: 12 months after receival at -20 °C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Coupling conditions: Standard conditions identical to normal nucleobases
Cleavage conditions: AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65 °C
Deprotection conditions: Dmt-ON protocol
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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