CFDA SE Cell Tracing Kit

Cell proliferation Microscopy Flow cytometry Cell viability and apoptosis
Cat. # Quantity Price Lead time
16231
3 vials
–   1 days
26231
15 vials
$230 1 days
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CFDA SE Cell Tracing Kit is used for fluorescent labeling and long-term tracing of cells.

CFDA SE ((5,6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore CFSE with emission in the green spectrum range (absorption max. at ~492 nm, emission max. at ~517 nm). CFSE interacts with cellular amines via its succinimidyl groups and covalently labels intracellular proteins.

CFDA SE is commonly used for in vivo and in vitro labeling of cells to analyze their proliferation as well as for cell tracking and motility assays. Inside the cell, CFDA SE shows little cytotoxicity, with minimal effects on the proliferative ability or biology of the cell. During proliferation, the label is partitioned approximately equally among the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions.

CFDA SE cell tracing kit contains single-use vials of dry dye and the required amount of high-quality anhydrous DMSO. It allows the preparation of small volumes of the working staining solution, which is convenient for conducting experiments and scaling them up without unnecessary loss of the dye.

CFDA SE cell tracing kit, 16231

CFDA absorbance and emission spectra

CFDA absorbance and emission spectra

Ready-to-use kit manual

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(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.

General properties

Storage conditions: Transportation: at RT for up to 3 weeks. Store at -20 °C.
MSDS: Download

Spectral properties

Excitation/absorption maximum, nm: 492
Emission maximum, nm: 517

Kit

Storage time in months: 12

References

  1. Girich, E.V.; Trinh, P.T.H; Nesterenko, L.E.; Popov, R.S.; Kim, N.Y.; Rasin, A.B.; Menchinskaya, E.S.; Kuzmich, A.S.; Chingizova, E.A.; Minin, A.S.; Ngoc, N.T.D.; Van, T.T.T.; Yurchenko, E.A.; Yurchenko, A.N.; Berdyshev, D.V. Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived Fungus Aspergillus sp. 1901NT-1.2.2. International Journal of Molecular Sciences, 2023, 24(9), 8150. doi: 10.3390/ijms24098150
  2. Silchenko, A.S.; Kalinovsky, A.I.; Avilov, S.A.; Popov, R.S.; Dmitrenok, P.S.; Chingizova, E.A.; Menchinskaya, E.S.; Panina, E.G.; Stepanov, V.G.; Kalinin, V.I.; Stonik, V.A. Djakonoviosides A, A1, A2, B1–B4 — Triterpene Monosulfated Tetra- and Pentaosides from the Sea Cucumber Cucumaria djakonovi : The First Finding of a Hemiketal Fragment in the Aglycones; Activity against Human Breast Cancer Cell Lines. International Journal of Molecular Sciences, 2023, 24(13), 11128. doi: 10.3390/ijms241311128
  3. Zhuravleva, O.I.; Chingizova, E.A.; Oleinikova, G.K.; Starnovskaya, S.S.; Antonov, A.S.; Kirichuk, N.N.; Menshov, A.S.; Popov, R.S.; Kim, N.Y..; Berdyshev, D.V.; Chingizov, A.R.; Kuzmich, A.S.; Guzhova, I.V.; Yurchenko, A.N.; Yurchenko, E.A. Anthraquinone Derivatives and Other Aromatic Compounds from Marine Fungus Asteromyces cruciatus KMM 4696 and Their Effects against Staphylococcus aureus. Marine Drugs, 2023, 21(8), 431. doi: 10.3390/md21080431
  4. Silchenko, A.S.; Kalinovsky, A.I.; Avilov, S.A.; Popov, R.S.; Chingizova, E.A.; Menchinskaya, E.S.; Zelepuga, E.A.; Panina, E.G.; Stepanov, V.G.; Kalinin, V.I.; Dmitrenok, P.S. Sulfated Triterpene Glycosides from the Far Eastern Sea Cucumber Cucumaria djakonovi : Djakonoviosides C 1 , D 1 , E 1 , and F 1 ; Cytotoxicity against Human Breast Cancer Cell Lines; Quantitative Structure–Activity Relationships. Marine Drugs, 2023, 21(12), 602. doi: 10.3390/md21120602
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