PreciseGreen® dsDNA Quantification Kit

PCR and qPCR Nucleic acid quantification

Cat. #	Quantity	Price	Lead time
1102-20	20 assays	–	1 days
1102-200 / 1 mL dye	200 assays	$450	1 days
B1102 / 10 x 100 uL dye	200 assays	$500	21 days

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Kit for quantitative measurement of double-stranded DNA in samples in the presence of RNA, proteins, and common contaminants. PreciseGreen® allows to quantitate as little as low pg/mL of dsDNA. The range of linearity for DNA concentration measurements
is 1 pg/μL – 5 ng/μL, so the sensitivity of this kit can be rated as Ultra High (UHS).

The kit contains PreciseGreen® dye, buffer, and DNA standard for the measurements. It is compatible with fluorometers, fluorescent plate readers, cuvette, and microvolume fluorometers.

Ready-to-use kit manual

PreciseGreen® dsDNA Quantification Kit manual

Calculator

dsDNA quantification calculator

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PreciseGreen dsDNA Quantification Reagent, 200×

PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

General properties

Storage conditions:	Store at temperature below 4°С. Do not freeze!
MSDS:	Download

Kit

Storage time in months:

References

Kusmierczyk, J.; Wiecek, M.; Bawelski, M.; Szygula, Z.; Rafa-Zablocka, K.; Kantorowicz, M.; Szymura, J. Pre-exercise cryotherapy reduces myoglobin and creatine kinase levels after eccentric muscle stress in young women. Frontiers in Physiology, 2024, 15, 1413949. doi: 10.3389/fphys.2024.1413949
Izzo, A.; Akol, I.; Villarreal, A.; Lebel, S.; Garcia-Miralles, M.; Cheffer, A.; Bovio, P.; Heidrich, S.; Vogel, T. Nucleophosmin 1 Cooperates with the Methyltransferase DOT1L to Preserve Peri-Nucleolar Heterochromatin Organization by Regulating H3K27me3 Levels and DNA Repeats Expression. Epigenetics & Chromatin, 2023, 16(1), 36. doi: 10.1186/s13072-023-00511-9
Psotta, C.; Chaturvedi, V.; Gonzalez-Martinez, J. F.; Sotres, J.; Falk, M. Portable Prussian Blue-Based Sensor for Bacterial Detection in Urine. Sensors, 2023, 23(1), 388. doi: 10.3390/s23010388
Sharma, A. K.; Fitieh, A. M.; Locke, A. J.; Ali, J. Y. H.; Ismail, I. H. Quantification of Protein Enrichment at Site-Specific DNA Double-Strand Breaks by Chromatin Immunoprecipitation in Cultured Human Cells. STAR Protocols, 2023, 4(1), 101917. doi: 10.1016/j.xpro.2022.101917