Protein Biotinylation Kit
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| BB21-1rxn
/ for 100 ug IgG
|
1 rxn
|
1138.80¥
|
21 days | |
| BB21-10rxn
/ for 10× 100 ug IgG
|
10 rxn
|
2839.70¥
|
21 days |
Protein Biotinylation Kit is a ready-to-use kit for covalent labeling of purified proteins, peptides, and antibodies with biotin using activated NHS-ester biotin. The kit provides fast, reproducible, and gentle biotinylation of primary amino groups (the ε-amino groups of lysine residues and N-terminal amino groups) without significantly affecting the protein’s biological activity.
The optimized protocol enables biotinylation in a short time and allows control over the degree of labeling by adjusting the reagent/protein ratio. The resulting biotinylated conjugates are fully compatible with streptavidin-biotin systems and with standard detection and affinity-immobilization methods.
Key Features
- One-step biotinylation of primary amino groups using NHS-biotin
- Fast reaction at physiological and slightly alkaline pH
- Suitable for most purified peptides, proteins, and antibodies
- Does not require prior protein modification
- Preserves native protein properties due to mild reaction conditions
- Compatible with ELISA, Western blotting, enzyme immunoassay, immunochemistry, flow cytometry, and affinity methods
Applications
- Preparation of biotinylated antibodies and antigens
- Streptavidin/avidin-based immunoassays
- Affinity immobilization of proteins
- Cellular and biochemical research
- Development of diagnostic reagents
We also produce a wide range of fluorescently labeled streptavidins for immunochemistry.
Ready-to-use kit manual
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General properties
| Storage conditions: | Store at temperature between 4 °C and 20 °C. Do not freeze! Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
Kit
| Storage time in months: | 12 |
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