7-AAD, far-red fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
4569-5mg 5 mg
$440
in stock
4569-10mg 10 mg
$704
in stock
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Items in stock will be shipped on 07/24/2025
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7-AAD (7-Aminoactinomycin D) is a fluorescent dye with a strong affinity for GC-rich regions of DNA. 7-AAD does not pass through the intact membrane of live cells, which is commonly used for detecting or excluding apoptotic and dead cells in flow cytometry and fluorescence microscopy. It is also used to stain DNA and nuclei in fixed and permeabilized cells and tissues.

7-AAD fluorescence spectra display a very large Stokes shift with maximum excitation at 488, 546, and 578 nm and maximum emission at 647 nm. 7-AAD is, therefore, compatible with most blue, green, and even many red fluorophores in multicolor applications.

Excitation and emission spectra of dsDNA complex with 7-AAD

Excitation and emission spectra of dsDNA complex with 7-AAD

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General properties

Appearance: purple powder
Molecular weight: 1270.45
CAS number: 7240-37-1
Molecular formula: C62H87N13O16
Solubility: DMSO, DMF, DCM, methanol
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 527 (complex)
Emission maximum, nm: 640 (complex)
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