7-AAD, far-red fluorescent nucleic acid stain

Nuclei Cell viability and apoptosis Microscopy Flow cytometry

Cat. #	Quantity	Price	Lead time	Buy this product
4569-5mg	5 mg	$440	in stock
4569-10mg	10 mg	$704	in stock

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7-AAD (7-Aminoactinomycin D) is a fluorescent dye with a strong affinity for GC-rich regions of DNA. 7-AAD does not pass through the intact membrane of live cells, which is commonly used for detecting or excluding apoptotic and dead cells in flow cytometry and fluorescence microscopy. It is also used to stain DNA and nuclei in fixed and permeabilized cells and tissues.

7-AAD fluorescence spectra display a very large Stokes shift with maximum excitation at 488, 546, and 578 nm and maximum emission at 647 nm. 7-AAD is, therefore, compatible with most blue, green, and even many red fluorophores in multicolor applications.

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Excitation and emission spectra of dsDNA complex with 7-AAD

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General properties

Appearance:	purple powder
Molecular weight:	1270.45
CAS number:	7240-37-1
Molecular formula:	C₆₂H₈₇N₁₃O₁₆
Solubility:	DMSO, DMF, DCM, methanol
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	527 (complex)
Emission maximum, nm:	640 (complex)

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.