AMCA azide

Azides Click chemistry AMCA

Cat. #	Quantity	Price	Lead time
3605-1mg	1 mg	$60	in stock
3605-5mg	5 mg	$110	in stock
3605-25mg	25 mg	$540	in stock
3605-50mg	50 mg	$885	in stock
3605-100mg	100 mg	$1050	in stock

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AMCA dye azide for сlick сhemistry conjugation with terminal alkynes via a copper-catalyzed click reaction or strained cyclooctynes via a copper-free click reaction.

AMCA (aminomethylcoumarin acetate) is one of the brightest blue fluorescent dyes. This fluorophore has a relatively large Stoke’s shift, high resistance to photobleaching, and pH-independent fluorescence from pH 4 to 10. AMCA is a widely used fluorophore for multiple-color labeling due to its minimal fluorescence overlap with green- and longer wavelength-emitting fluorescent dyes.

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Absorption and emission spectra of AMCA

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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General properties

Appearance:	yellow powder
Molecular weight:	315.33
Molecular formula:	C₁₅H₁₇N₅O₃
Solubility:	in DMSO, DMF
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	348
ε, L⋅mol⁻¹⋅cm⁻¹:	17400
Emission maximum, nm:	435
Fluorescence quantum yield:	0.91
CF₂₆₀:	0.16
CF₂₈₀:	0.13

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.