ROX alkyne, 5-isomer

Cat. # Quantity Price Lead time
A12B0 1 mg $110 in stock
B12B0 5 mg $165 in stock
C12B0 10 mg $210 in stock
D12B0 25 mg $285 in stock
E12B0 50 mg $495 in stock
F12B0 100 mg $1190 in stock
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ROX alkyne is an alkyne derivative of the bright red-emitting dye ROX (Rhodamine X, Rhodamine 101). Due to its quantum yield approaching unity, ROX is a popular dye for qPCR and microscopy applications. It is used for fluorescent labeling of azide-containing biomolecules via copper-catalyzed click reaction. This product is a pure 5-isomer of ROX.

ROX is prone to oxidation; therefore, its derivatives should preferably be stored in an inert atmosphere.

Absorption and emission spectra of ROX

Absorption and emission spectra of ROX

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FAM DBCO, 6-isomer

Cyclooctyne derivative of fluorescein (FAM) for copper-free click chemistry reaction with azides. Pure 6-isomer of fluorescein.

TAMRA alkyne, 6-isomer

Terminal alkyne for copper-catalyzed click chemistry. Tetramethylrhodamine (TAMRA) is a rhodamine dye which is popular in qPCR and other applications. TAMRA is a also a FRET acceptor for FAM.

General properties

Appearance: purple solid
Mass spec M+ increment: 571.3
Molecular weight: 571.66
CAS number: 2264016-88-6
Molecular formula: C36H33N3O4
Solubility: good in DMSO, DMF, methanol, ethanol
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00
CF260: 0.62
CF280: 0.49
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