AF 555 alkyne
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1K8B0 | 1 mg |
$189.00
|
in stock | |
| 2K8B0 | 5 mg |
$599.00
|
in stock | |
| 4K8B0 | 25 mg |
$1790.00
|
in stock |
AF 555 alkyne is a yellow fluorescent dye containing a terminal alkyne group for bioorthogonal conjugation via copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The dye reacts selectively with azide-modified biomolecules, forming stable triazole linkages under mild aqueous conditions.
AF 555 alkyne absorbs at ~552 nm and emits bright fluorescence at ~566 nm. The dye is characterized by high brightness, good photostability, and high water solubility, making it suitable for fluorescence microscopy, flow cytometry, and high-content screening. Its excitation profile is compatible with standard 532–561 nm laser lines.
The reagent is commonly used for post-labeling of azide-functionalized proteins, glycans, nucleic acids, lipids, and other biomolecules introduced by metabolic labeling or chemical modification.
Absorption and emission spectra of AF 555
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General properties
| Appearance: | dark red powder |
| Molecular weight: | 998.36 |
| Molecular formula: | C38H46K3N3O13S4 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 152000 |
| Emission maximum, nm: | 566 |
| Fluorescence quantum yield: | 0.14 |
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