Ac4ManNAz (N-Azidoacetylmannosamine-tetraacylated)
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The tetraacetylated N-Azidoacetyl-mannosamine (Ac4ManNAz) is an azide-labeled monosaccharide that provides a highly specific tool for studying glycoproteins through metabolic labeling in vivo and subsequent chemoselective ligation.
Ac4ManNAz is cell-permeable unnatural sugar that is intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylmannosamine (ManNAc).
The resulting azide-contained glycoprotein can be detected via Cu(I)-catalyzed (CuAAC) or copper-free (SPAAC) click reaction with either fluorescent-labeled alkynes /cycloalkynes or biotin-alkyne .
The recommended concentration for cell labeling is 25-75 μM, and this concentration range may be a starting point for an individual experiment setup.
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General properties
Appearance:
white solid
Molecular weight:
430.37
CAS number:
361154-30-5
Molecular formula:
C16 H22 N4 O10
Solubility:
Soluble to 100 mM in DMSO
Quality control:
NMR 1 H and HPLC-MS (95+%)
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:
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Product specifications
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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