AF 555 DBCO

Cycloalkynes AF 555 Copper-free click chemistry
Cat. # Quantity Price Lead time
1K8F0 1 mg $140 in stock
2K8F0 5 mg $450 in stock
4K8F0 25 mg $1460 in stock
5K8F0 50 mg $1890 in stock
6K8F0 100 mg $2990 in stock
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 555 is a hydrophilic fluorophore with high fluorescence quantum yield and high photostability, an alternative to tetramethylrhodamine (TAMRA, TMR) or Cyanine3 dyes.

AF 555 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

AF 555 DBCO, 2K8F0
AF 555 DBCO, 2K8F0
AF 555 DBCO, 2K8F0
Structure of AF 555 DBCO
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Absorption and emission spectra of AF 555

Absorption and emission spectra of AF 555

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General properties

Appearance: purple powder
Molecular weight: 1279.71
Molecular formula: C56H65K3N4O15S4
Solubility: water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
ε, L⋅mol−1⋅cm−1: 152000
Emission maximum, nm: 566
Fluorescence quantum yield: 0.14
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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