Annexin V-AF 488 conjugate

Cell viability and apoptosis Flow cytometry Microscopy

Cat. #	Quantity	Price	Lead time	Buy this product
11515	1 ug	–	in stock
21515	5 ug	$119	in stock

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Annexin V (or Annexin A5) belongs to the phospholipid-binding Annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine, which migrates from the inner to the outer leaflet of the plasma membrane during the early stages of apoptosis.

This Annexin V is a lyophilized conjugate with AF 488, a bright, photostable green fluorophore with spectral characteristics similar to FITC (absorption max. at 495 nm, emission max. at 519 nm).

Annexin V-AF 488 staining alone does not segregate the populations of apoptotic and necrotic cells. For this, additional staining with nuclear dyes that do not penetrate living cells — with propidium iodide or YODi-3 is required. For this purpose, you can also use our ready-to-use Annexin V-AF 488 apoptosis detection kit.

Dissolve the content of the Annexin V-AF lyophilized conjugate tube in 50 µL (11515) or in 250 µL (21515) of deionized water.

Important! The dissolved conjugate should be stored protected from light at 2-8 °C. In solution, the conjugate is stable for a month. For longterm use, it is recommended to prepare aliquots and store them at −20 °C. Avoid re-freezing!

Absorption and emission spectra of AF 488

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General properties

Appearance:	dark orange solid
Solubility:	good in water
Storage conditions:	Store at -20 °С 9 months. Transportation: at room temperature for 1 week.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	495
ε, L⋅mol⁻¹⋅cm⁻¹:	71800
Emission maximum, nm:	519
Fluorescence quantum yield:	0.91
CF₂₆₀:	0.16
CF₂₈₀:	0.10

Product citations

Fayzullina, D.; Yakushov, S.; Kantserova, K.; Belyaeva, E.; Aniskin, D.; Tsibulnikov, S.; Fayzullina, N.; Kalinin, S.; Romantsova, O.; Timashev, P.S.; Schroeder, B.A.; Ulasov, I.V. Carbonic Anhydrase Inhibitors Induce Ferroptosis through Inhibition of AKT/FTH1 Signaling in Ewing Sarcoma Tumor Cells. Cancers, 2023, 15(21), 5225. doi: 10.3390/cancers15215225

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.