BDP® 576/589 tetrazine

Tetrazines BDP® 576/589 Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
1G4E0	1 mg	$110	in stock
2G4E0	5 mg	$290	in stock
3G4E0	10 mg	$450	in stock
4G4E0	25 mg	$690	in stock
5G4E0	50 mg	$1270	in stock
6G4E0	100 mg	$1990	in stock

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Because of its relatively long excited-state lifetime (about 5 nanoseconds), BDP 576/589 dye can be used in various methods based on measuring fluorescence lifetime. Similarly to other dyes of the BDP family, BDP 576/589 has strong hydrophobic properties and is suitable for labeling non-polar and lypophilic biomolecules and their subsequent visualization by fluorescent microscopy, including two-photon microscopy.

This reagent is a tetrazine derivative that can be conjugated with various strained dienophiles such as trans-cyclooctenes and cyclopropenes. This reaction (TCO ligation) is considered to be one of the best bioconjugation reactions because it is very fast and selective in physiological conditions and does not require additional catalyzers, so it is not toxic in vitro and in vivo.

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BDP 576/589 absorbance and emission spectra

General properties

Appearance:	dark colored solid
Mass spec M+ increment:	501.16
Molecular weight:	529.17
Molecular formula:	C₂₆H₂₃N₈BF₂O
Solubility:	good in polar organic solvents
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	580
ε, L⋅mol⁻¹⋅cm⁻¹:	98000
Emission maximum, nm:	592
Fluorescence quantum yield:	0.13
CF₂₆₀:	0.32
CF₂₈₀:	0.35

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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.