BDP® FL L-Cystine
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BDP FL L-cystine is a symmetric disulfide for reversible thiol-specific labeling of thiolated oligonucleotides, proteins, and cells.
BDP FL L-cystine consists of two BDP FL molecules connected via a disulfide bridge between two cysteine residues. In the dimeric disulfide state, the fluorescence of the dye is quenched.
Like other symmetric disulfides, BDP FL L-cystine undergoes a thiol-disulfide interchange reaction. Reduction of the disulfide bridge releases the monomeric dye molecule and results in fluorescence in the green region of the spectrum.
Absorption and emission spectra of BDP FL
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General properties
Appearance:
orange powder
Molecular weight:
788.46
Molecular formula:
C34 H38 B2 F4 N6 O6 S2
IUPAC name:
(2R)-3-[[(2R)-2-carboxy-2-[3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)propanoylamino]ethyl]disulfanyl]-2-[3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)propanoylamino]propanoic acid
Quality control:
NMR 1 H and HPLC-MS (95+%)
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:
Download
Product specifications
Spectral properties
Excitation/absorption maximum, nm:
503
ε, L⋅mol−1 ⋅cm−1 :
92000
Emission maximum, nm:
509
Fluorescence quantum yield:
0.97
CF260 :
0.015
CF280 :
0.027
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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