CCK-8, cell proliferation assay

Cat. # Quantity Price Lead time
23972
/ lyophilized form
100 rxn
$45.00
in stock
33972
/ lyophilized form
500 rxn
$159.00
in stock
43972
/ lyophilized form
1000 rxn
$275.00
in stock
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CCK-8 (Cell Counting Kit 8) is a sensitive colorimetric assay used in biomedical research to determine cell viability, proliferation, and cytotoxicity. It works by employing the highly water-soluble tetrazolium salt, WST-8, which is reduced by intracellular dehydrogenases in live cells into a water-soluble, orange-colored formazan dye. The intensity of this color, measured by a microplate reader at a specific wavelength (around 450 nm), is directly proportional to the number of metabolically active cells.

CCK-8 Assay

The CCK-8 assay offers ease of use, minimal toxicity, and enhanced sensitivity compared to tests based on other tetrazolium salts such as MTT, XTT, MTS, or WST-1.

This product is a ready-to-use lyophilized mixture containing the tetrazolium salt WST-8 and the electron mediator 1-Methoxy PMS in the required concentrations.

Excitation spectrum of WST-8

Excitation spectrum of WST-8

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General properties

Appearance: pink foam
Solubility: water
Quality control: NMR 1H and HPLC-MS (95+%), functional test
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 458
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