Unfortunately this product is not available in your region.D-Luciferin, Potassium Salt
D-luciferin is a low-molecular-weight substrate for the firefly (Photinus pyralis) luciferase enzyme. The ATP-dependent oxidation of D-luciferin by luciferase is accompanied by intense chemiluminescence at 560 nm.
Luciferase is encoded by the luc gene, which is widely used as a reporter gene for the genetic modification of cells and organisms. When D-luciferin is administered, it is taken up by cells, and the appearance of luminescence indicates the presence and activity of the luciferase. Because most organisms do not naturally synthesize luciferase, the background signal is virtually absent, making this reporter system extremely sensitive.
The luciferase/luciferin system is used to study gene expression, noninvasively monitor tumor growth, metastasis, or the fate of transplanted stem cells in living animals over time, visualize infections, measure ATP levels in cells, and assess cell viability.
For in vivo experiments, the standard working dose is 100-150 mg/kg of mouse body weight administered intraperitoneally. For in vitro assays, the working concentration is typically 0.1-1.0 mM. The signal intensity and kinetics depend on pH, ATP and Mg2+ concentrations, temperature, and the permeability of cell membranes to the substrate.
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General properties
| Appearance: | grey solid |
| Molecular weight: | 318.42 |
| CAS number: | 115144-35-9 (potassium salt) |
| Molecular formula: | C11H7KN2O3S2 |
| Solubility: | well soluble in methanol, DMSO; moderately soluble in water (at pH > 6.5); poorly soluble in non-polar organic solvents |
| Quality control: | NMR 1H and HPLC-MS (99+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
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