DBCO-NHS ester

NHS esters Cycloalkynes Protein labeling Crosslinking Copper-free click chemistry
Cat. # Quantity Price Lead time
34720 10 mg $80 in stock
44720 25 mg $110 in stock
54720 50 mg $135 in stock
64720 100 mg $178 in stock
94720 1 g $970 in stock
K4720 5 g $2990 in stock
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

Dibenzocyclooctyne (ADIBO, DBCO) is one of the most reactive cycloalkynes for strain-promoted alkyne azide cycloaddition (SPAAC) — a copper-free click chemistry reaction.

DBCO reacts instantly with azides. The reaction rate is much higher than that of copper-catalyzed reaction, and reactions with many other cyclooctynes. Unlike some other cyclooctynes, DBCO does not react with tetrazines — this allows to carry out orthogonal conjugation of azides with DBCO, and trans-cyclooctenes with tetrazines.

This amine-reactive NHS ester provides easy attachment of the reactive moiety to almost any primary or secondary amine group, such as protein, peptide, or small molecule amine.

DBCO-NHS ester, 64720
DBCO-NHS ester, 64720
DBCO-NHS ester, 64720
DBCO (ADIBO) NHS ester structure
Download in
ChemDraw format

Recommended protocol

Calculator

Customers also purchased with this product

AmdU (5-Azidomethyl-2'-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.

3'-Azido-modifier CPG 1000A

Controlled pore glass solid support for DNA/RNA synthesis with azide modification (pore size 1000 Å) for the synthesis of long modified oligonucleotides with 3'-azide modification.

BDP® 493/503 lipid stain

BDP 493/503 is a lipophilic green fluorescent dye for the staining and tracing of cell membranes, lipids, and oils.

General properties

Appearance: off white solid
Mass spec M+ increment: 315.1
Molecular weight: 430.45
CAS number: 1384870-47-6
Molecular formula: C25H22N2O5
IUPAC name: 6-{2-Azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl}-6-oxohexanamide
Solubility: good in DCM, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Product citations

  1. Mitchell, M.I.; Ben‐Dov, I.Z.; Ye, K.; Liu, C.; Shi, M.; Sadoughi, A.; Shah, C.; Siddiqui, T.; Okorozo, A.; Gutierrez, M.; Unawane, R.; Biamonte, L.; Parihk, K.; Spivack, S.; Loudig, O. Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed. Journal of Extracellular Vesicles, 2024, 13(4), e12440. doi: 10.1002/jev2.12440
  2. Mitchell, M.I.; Ben-Dov, I.Z.; Liu, C.; Wang, T.; Hazan, R.B.; Bauer, T.L.; Zakrzewski, J.; Donnelly, K.; Chow, K.; Ma, J.; Loudig, O. Non-invasive detection of orthotopic human lung tumors by microRNA expression profiling of mouse exhaled breath condensates and exhaled extracellular vesicles. Extracellular Vesicles and Circulating Nucleic Acids, 2024, 5(1), 138-64. doi: 10.20517/evcna.2023.77
  3. Mitchell, M.I.; Khalil, M.; Ben-Dov, I.Z.; Alverez-Perez, J.; Illsley, N.P.; Zamudio, S.; Al-Khan, A.; Loudig, O. Customizing EV-CATCHER to Purify Placental Extracellular Vesicles from Maternal Plasma to Detect Placental Pathologies. International Journal of Molecular Sciences, 2024, 25(10), 5102. doi: 10.3390/ijms25105102
  4. Gabold, B.; Adams, F.; Brameyer, S.; Jung, K.; Ried, C. L.; Merdan, T.; Merkel, O. M. Transferrin-Modified Chitosan Nanoparticles for Targeted Nose-to-Brain Delivery of Proteins. Drug Deliv. and Transl. Res., 2023, 13(3), 822–838. doi: 10.1007/s13346-022-01245-z
Show more (8)
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/2S
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.