DusQ 1 CPG 500
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11960 | 100 mg | – | in stock | |
| 31960 | 500 mg |
$530
|
in stock | |
| 41960 | 1 g |
$990
|
in stock | |
| 51960 | 5 g | please inquire | in stock |
This modified support with a pore size of 500 Å is intended for the synthesis of oligonucleotides of up to 50 bases in length modified with non-fluorescent DusQ 1 quencher at the 3’ end.
DusQ 1 dark quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It can be used for combined quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™, CAL Fluor™ Gold 540, and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
Absorption spectrum of DusQ 1
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Ready-to-use catalytic buffer containing сopper(II) and TBTA ligand. It is suitable for click chemistry modification of nucleic acids and small molecules.Universal CPG type I, 500A
This universal support is intended for the automated synthesis of all types of oligonucleotides. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases in length.Cyanine3.5 azide
Cyanine3.5 azide for biomolecule labeling through click chemistry.General properties
| Appearance: | purple beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
| CF260: | 0.17 |
| CF280: | 0.10 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 70−80 |
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