DusQ 1 CPG 1000
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14960 | 100 mg | – | in stock | |
| 34960 | 500 mg |
$530
|
in stock | |
| 44960 | 1 g |
$990
|
in stock | |
| 54960 | 5 g | please inquire | in stock |
This support with a pore size of 1000 Å is intended for the automated synthesis of oligonucleotides of up to 120 bases in length modified with DusQ1 dark quencher at the 3’ end.
Non-fluorescent DusQ1 quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It is suitable for quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™ and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
Absorption spectrum of DusQ 1
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Alkyne phosphoramidite, 5'-terminal
Alkyne phosphoramidite for the synthesis of oligonucleotides ready for click chemistry.Universal CPG type II, 1000A
This universal support is intended for automated synthesis of oligonucleotides to accelerate the dephosphorylation process during deblocking. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.General properties
| Appearance: | red beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
| CF260: | 0.17 |
| CF280: | 0.10 |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 30−50 |
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