DusQ 1 CPG 1000

Cat. # Quantity Price Lead time
14960 100 mg –   in stock
34960 500 mg $530 in stock
44960 1 g $990 in stock
54960 5 g please inquire in stock
Found better price? Let us know and we will propose the way forward!

This support with a pore size of 1000 Å is intended for the automated synthesis of oligonucleotides of up to 120 bases in length modified with DusQ1 dark quencher at the 3’ end.

Non-fluorescent DusQ1 quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It is suitable for quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™ and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.

Usage

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.

Absorption spectrum of DusQ 1

Absorption spectrum of DusQ 1

Customers also purchased with this product

ROX NHS ester, 6-isomer

Pure 6-isomer of ROX (Rhodamine X, R101) NHS-ester for amine labeling.

JOE azide, 5-isomer

JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for сlick chemistry

TAMRA azide, 5-isomer

Azide derivative of TAMRA fluorescent dye for сlick chemistry.

General properties

Appearance: red beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 27300
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Pore size, Å: 1000
Typical loading, umol/g: 30−50
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.