AF 384 (PEP) azide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A4530 | 1 mg | $110 | in stock | |
B4530 | 5 mg | $210 | in stock | |
C4530 | 10 mg | $310 | in stock | |
D4530 | 25 mg |
$410
|
in stock | |
E4530 | 50 mg |
$695
|
in stock |
Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, AF 384 (PEP) fluorescence is more red-shifted
PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.
This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and click chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.
Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

Recommended protocol
Calculator
Customers also purchased with this product
3-Maleimidopropionic acid NHS-ester
Bifunctional crosslinker with an amine-reactive succinimidyl ester and a thiol-reactive maleimide group.EdU (5-Ethynyl-2'-deoxyuridine)
EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.BDP® FL amine
Amino derivative of BDP FL dye, borondipyrromethene dye for FAM (fluorescein) 488 nm channel.General properties
Appearance: | off white / yellowish solid |
Molecular weight: | 544.64 |
CAS number: | 1807521-02-3 |
Molecular formula: | C34H32N4O3 |
Solubility: | soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile |
Quality control: | NMR 1H (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 293; 362; 384 |
ε, L⋅mol−1⋅cm−1: | 46300 |
Emission maximum, nm: | 389 |
Fluorescence quantum yield: | 0.64 |
Product citations
- Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
