Pyrenebutyric acid NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 63520 | 100 mg | $110 | in stock |
Pyrene is a polyaromatic hydrocarbon which exhibits fluorescence in blue range of the spectrum. When two pyrene residues are closely spaced, excimer fluorescence is observed at longer wavelength. Therefore, pyrene is useful as a proximity label.
Pyrene exhibits affinity to graphite, and can be used as an anchor group for graphite. This reagent can be conjugated with biomolecules for their subsequent immobilization on graphite surfaces.
This NHS ester allows to label biomolecules with amino groups, such as proteins, peptides, amino-DNA and other molecules.
Pyrene is also a FRET donor for perylene.
Absorption and emission spectra of pyrene fluorophore
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General properties
| Appearance: | off-white solid |
| Mass spec M+ increment: | 270.1 |
| Molecular weight: | 385.41 |
| CAS number: | 114932-60-4 |
| Molecular formula: | C24H19NO4 |
| IUPAC name: | 1-Pyrenebutanoic acid, 2,5-dioxo-1-pyrrolidinyl ester |
| Solubility: | good in DCM, chloroform, moderate in DMF, DMSO, low in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 343; 326; 313; 276; 265; 242; 234 |
| Emission maximum, nm: | 377; 397 |
Product citations
- Nasimi, H.; Madsen, J. S.; Zedan, A. H.; Malmendal, A.; Osther, P. J. S.; Alatraktchi, F. A. Identification of Early Stage and Metastatic Prostate Cancer Using Electrochemical Detection of Beta-2-Microglobulin in Urine Samples from Patients. Scientific Reports, 2023, 13(1), 10658. doi: 10.1038/s41598-023-37886-4
- Nekrasov, N.; Jaric, S.; Kireev, D.; Emelianov, A.V.; Orlov, A.V.; Gadjanski, I.; Nikitin, P.I.; Akinwande, D.; Bobrinetskiy, I. Real-time detection of ochratoxin A in wine through insight of aptamer conformation in conjunction with graphene field-effect transistяor. Biosensors and Bioelectronics, 2022, 200, 113890. doi: 10.1016/j.bios.2021.113890
- Epanchintseva, A. V.; Gorbunova, E. A.; Ryabchikova, E. I.; Pyshnaya, I. A.; Pyshnyi, D. V. Effect of Fluorescent Labels on DNA Affinity for Gold Nanoparticles. Nanomaterials, 2021, 11(5), 1178. doi: 10.3390/nano11051178
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