Pyrene phosphoramidite dU

Oligonucleotide synthesis Pyrene Phosphoramidites

Cat. #	Quantity	Price	Lead time
11460	100 mg	$240	in stock
21460	250 mg	$460	in stock
41460	1 g	$890	in stock
61460	5 g	please inquire	in stock

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Pyrene is a polycyclic aromatic hydrocarbon that is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on the fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about the site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.

With this phosphoramidite, pyrene can be introduced into DNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows attaching pyrene fragment to 5', internal, or 3' position (using universal support).

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

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Absorption and emission spectra of pyrene fluorophore

General properties

Appearance:	yellowish foam
Molecular weight:	955.04
CAS number:	199920-17-7
Molecular formula:	C₅₇H₅₅N₄O₈P
Solubility:	good in dichloromethane and acetonitrile
Quality control:	NMR ¹H (95%), ³¹P, HPLC-MS
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	260; 282; 365; 392
ε, L⋅mol⁻¹⋅cm⁻¹:	12600; 21900; 16000; 14200
Emission maximum, nm:	460
Fluorescence quantum yield:	~0.1

Oligo synthesis details

Diluent:

acetonitrile

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.