ROX reference dye for qPCR
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
31110 | 500 uL | – | in stock | |
41110 | 1 mL | $35 | in stock | |
51110 | 25 mL | $265 | in stock | |
61110 | 50 mL | $345 | in stock |
This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as dsGreen or TaqMan probes. It also allows for correcting pipetting errors and instrumental drift, such as changes in lamp intensity output over time.
The dye contains 25 μM solution of rhodamine 101 reference dye in 10 mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.
ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.
Customers also purchased with this product
Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.PreciseGreen dsDNA Quantification Reagent, 200×
PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.BDP TR alkyne
BDP TR is a fluorophore for ROX channel. This borondipyrromethene dye is very bright, and resistant to oxidation.General properties
Appearance: | pink solution |
Quality control: | functional test in RT PCR, UV-Vis abs |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 570 |
Emission maximum, nm: | 591 |
Product citations
- Bartlett, C.; Langsjoen, J.; Cheng, Q.; Yingling, A. V.; Weiss, M.; Bradfute, S.; Perkins, D. J.; Hurwitz, I. COVID-19 Global Pandemic Planning: Presence of SARS-CoV-2 Fomites in a University Hospital Setting. Exp Biol Med (Maywood), 2021, 246(18), 2039–2045. doi: 10.1177/15353702211024597
- Zhuo, X.-Z.; Bai, K.; Wang, Y.; Liu, P.; Xi, W.; She, J.; Liu, J. Long-chain non-coding RNA-GAS5 / hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2. Bioscience Reports, 2021, 41(9), BSR20202232. doi: 10.1042/BSR20202232
- Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038