SIMA azide, 6-isomer

Azides SIMA (Dichloro-diphenyl-fluorescein) Click chemistry

Cat. #	Quantity	Price	Lead time
AE230	1 mg	$110	in stock
BE230	5 mg	$180	in stock
CE230	10 mg	$310	in stock
DE230	25 mg	$410	in stock
EE230	50 mg	$695	in stock
FE230	100 mg	$1190	in stock

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SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.

SIMA azide is used to produce fluorescently labeled primers and hybridization probes for quantitative PCR. Oligonucleotides with a SIMA label can be easily generated via azide-alkyne cycloaddition between SIMA azide and alkyne-containing oligonucleotide.

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Absorption and emission spectra of SIMA

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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General properties

Appearance:	orange powder
Molecular weight:	679.52
Molecular formula:	C₃₆H₂₄Cl₂N₄O₆
Solubility:	good in DMSO, DMF
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	531
ε, L⋅mol⁻¹⋅cm⁻¹:	92300
Emission maximum, nm:	555
Fluorescence quantum yield:	0.63
CF₂₆₀:	0.57
CF₂₈₀:	0.18

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.