SIMA carboxylic acid, 6-isomer

Cat. # Quantity Price Lead time
2E290 5 mg $125 21 days
4E290 25 mg $225 21 days
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SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.

SIMA carboxylic acid is a non-reactive form of SIMA dye that can be used as a reference standard in experiments involving SIMA dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC.

Absorption and emission spectra of SIMA

Absorption and emission spectra of SIMA

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ROX carboxylic acid, 6-isomer

Derivative of ROX (Rhodamine X, Rhodamine 101) fluorophore with non-activated free carboxylic acid group. This reagent is a pure 6-isomer.

General properties

Appearance: orange powder
Molecular weight: 597.41
Molecular formula: C33H18Cl2O7
Solubility: good in DMSO, DMF, methanol, basic solutions, limited in acetonitrile
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 531
ε, L⋅mol−1⋅cm−1: 92300
Emission maximum, nm: 555
Fluorescence quantum yield: 0.63
CF260: 0.57
CF280: 0.18
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