AF 594 streptavidin
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A68S0 | 100 ug | – | in stock | |
| 168S0 | 1 mg | $185 | in stock |
Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.
Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.
This streptavidin is a lyophilized conjugate with AF 594, a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm).
The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.
Absorption and emission spectra of AF 594
Recommended protocol
Calculator
Customers also purchased with this product
FAM-11-dUTP, 6-isomer
FAM-derivative of dUTP with green emission for DNA labeling.AF 594 NHS ester
Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomerBDP® TMR ceramide
Fluorescent derivative of sphingosine with emission in the orange spectrum region for visualization of the Golgi apparatus in cells.General properties
| Appearance: | purple solid |
| Solubility: | good in water |
| Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
Your cart is empty
$ 
