sulfo-Cyanine5.5 NHS ester
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
17320 | 1 mg | $125 | in stock | |
27320 | 5 mg | $325 | in stock | |
47320 | 25 mg |
$850
|
in stock | |
57320 | 50 mg |
$1490
|
in stock | |
67320 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine5.5 NHS ester is an amine-reactive activated ester of sulfonated far-red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in a purely aqueous environment or without any significant additions of organic co-solvents.
The dye is well-suitable for non-invasive in vivo NIR imaging and applications requiring low fluorescent background.
Absorption and emission spectra of sulfo-Cyanine5.5
![Absorption and emission spectra of sulfo-Cyanine5.5](https://static.lumiprobe.us/img/spectra/sulfo-Cy55_absorption_emission.png)
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NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.General properties
Appearance: | dark blue solid |
Mass spec M+ increment: | 884.15 |
Molecular weight: | 1114.37 |
CAS number: | 2419286-92-1 |
Molecular formula: | C44H42N3K3O16S4 |
Solubility: | good in water, DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 675 |
ε, L⋅mol−1⋅cm−1: | 211000 |
Emission maximum, nm: | 694 |
Fluorescence quantum yield: | 0.21 |
CF260: | 0.09 |
CF280: | 0.11 |
Product citations
- Yao, P.; Liu, R.; Broginni, T.; Thunemann, M.; Kleinfeld, D. Guide to the Construction and Use of an Adaptive Optics Two-Photon Microscope with Direct Wavefront Sensing. bioRxiv, 2023, preprint. doi: 10.1101/2023.01.24.525307
- Zhou, L.; He, F.; Xiang, X.; Dong, C.; Xiang, T.; Li, X.; Li, H.; Bu, L.; Wang, Y.; Ma, X. Radioactive and Fluorescent Dual Modality Cysteine Cathepsin B Activity-Based Probe for Cancer Theranostics. Mol. Pharmaceutics, 2023, 20(7), 3539–3548. doi: 10.1021/acs.molpharmaceut.3c00148
- Shevchuk, A. I.; Svinko, V. O.; Smirnov, A. N.; Solovyeva, E. V. SERS Study of Cyanine Dyes: Optimization of Metal Core and Molecular Label Choice for Plasmonic Nanotags. Dyes and Pigments, 2023, 216, 111329. doi: 10.1016/j.dyepig.2023.111329.
- Svinko, V. O.; Smirnov, A. N.; Shevchuk, A. I.; Demenshin, A. I.; Smirnov, A. A.; Solovyeva, E. V. Comparative Study of Fluorescence Core-Shell Nanotags with Different Morphology of Gold Core. Colloids and Surfaces B: Biointerfaces, 2023, 226, 113306. doi: 10.1016/j.colsurfb.2023.113306
Cy® is a trademark of GE Healthcare.
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