ssGreen® RNA Gel Staining Solution, 10,000×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A2510 | 50 uL | – | in stock | |
| 12510 | 500 uL | $385 | in stock |
ssGreen® is one of the most sensitive stains for post-electrophoresis staining of RNA and single-stranded DNA (ssDNA) in agarose or polyacrylamide gels. The fluorescence quantum yield of the ssGreen/RNA complex is more than 7× higher than that of the ethidium bromide/RNA complex. Although ssGreen is not selective for RNA staining, the dye exhibits about 1.5× greater quantum yield when bound to RNA than double-stranded DNA, which makes it unique among all nucleic acid dyes.
ssGreen staining is compatible with denaturing gels. On agarose/formaldehyde and polyacrylamide/urea gels, the sensitivity of ssGreen is slightly reduced but still superior to that of ethidium bromide. Staining agarose/formaldehyde gels with ssGreen does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1%–0.3% SDS is included in prehybridization and hybridization buffers.
Staining gels with ssGreen has fewer steps than those with ethidium bromide. Because the fluorescence of ssGreen/RNA complexes is not quenched by formaldehyde or urea, there is no need to wash these denaturants out of gels before staining. Also, ssGreen stain has a low intrinsic fluorescence, allowing gel viewing and photographing without preliminary removing unbound dye.
Excitation and emission spectra of RNA complex with ssGreen
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| Appearance: | dark orange solution |
| Molecular weight: | 581.52 |
| CAS number: | 172827-25-7 |
| Molecular formula: | C28H28IN3OS |
| Quality control: | UV-Vis abs |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 483 (complex) |
| Emission maximum, nm: | 518 (complex) |
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