TET phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| C8160 | 100 mg | – | in stock | |
| D8160 | 250 mg | $197 | in stock | |
| F8160 | 1 g |
$575
|
in stock | |
| G8160 | 5 g |
$2265
|
in stock | |
| H8160 | 10 g | please inquire | in stock |
TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.
TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).
TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).
5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via PCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM® 310 Genetic Analyzer.
Recommendations for using the reagent:
Condensation: 3 min.
Deprotection: standard conditions with 25% ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 hours at 55°C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (10 min at 65°C).
Absorption and emission spectra of TET
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VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.General properties
| Appearance: | white solid foam |
| Molecular weight: | 981.72 |
| CAS number: | 877049-90-6 |
| Molecular formula: | C46H54N3Cl4O10P |
| IUPAC name: | 2',4,7,7'-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate) |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 100000 |
| Emission maximum, nm: | 535 |
| Fluorescence quantum yield: | 0.47 |
| CF260: | 0.17 |
| CF280: | 0.09 |
Oligo synthesis details
| Diluent: | anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week). |
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