Universal CPG type II, 500A
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3219-1g | 1 g | $45 | in stock | |
| 3219-10g | 10 g |
$410
|
in stock | |
| 3219-100g | 100 g | please inquire | 21 days |
The Universal CPG type II, 500A is used for oligonucleotides synthesis to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking due to a rigid bicyclic molecule on the support.
The Universal CPG type II, 500A is suitable for use in harsh conditions and makes cleavage and deprotection with anhydrous ammonia gas-phase, ammonium hydroxide/methylamine (AMA) mixture or other basic reagents faster compared to universal Controlled Pore Glass (CPG) supports. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 120 bases. For oligos up to 120 bases universal support 1000 Å can be used.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 2 hours at 80
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HEX azide, 6-isomer
Azide of green-yellow emitting HEX dye for conjugation with oligonucleotides via [3+2]-dipolar cycloaddition.General properties
| Appearance: | white powder |
| Quality control: | loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonium hydroxide 2 hours at 80 °C or AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 15 minutes at 65 °C |
| Deprotection conditions: | identical to protected nucleobases |
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