AF 488 tetrazine, 5-isomer

Tetrazines AF 488 Copper-free click chemistry
Cat. # Quantity Price Lead time
118E0 1 mg $140 21 days
218E0 5 mg $450 21 days
418E0 25 mg $1460 21 days
518E0 50 mg $1890 21 days
618E0 100 mg $2640 21 days
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AF 488 is a water-soluble green-fluorescent dye with high fluorescence quantum yield and high photostability. The dye is similar to FITC in spectral characteristics (absorption max. at 495 nm, emission max. at 519 nm) and is not sensitive to pH changes within the range from 4 to 10. This reagent is a pure 5-isomer.

This AF 488 derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick, specific and suitable for in vitro cell labeling.

Structure of AF 488 tetrazine, 5-isomer
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Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: deep orange powder
Molecular weight: 817.88
Molecular formula: C37H37N8O10S2-
Solubility: good in methanol, DMSO, DMF, and water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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