DusQ® 2 dT phosphoramidite
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
1451-100mg | 100 mg | $340 | in stock | |
1451-500mg | 500 mg |
$1390
|
in stock | |
1451-1g | 1 g |
$2290
|
in stock |
DusQ® 2 dT phosphoramidite is a modified oligo synthesis reagent bearing completely non-fluorescent quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 2 has large extinction coefficient, efficiently quench fluorescence for dyes emitting in the far red region, such as Cyanine5.
DusQ 2 is typically used to construct qPCR probes with a quencher moiety. Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 2 has a absorption spectrum in of red and near-infrared range and can be paired with reporter dyes that emit in the range 560–670 nm, e.g. TAMRA, ROX, Cyanine3, and Cyanine5.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
Customers also purchased with this product
Alkyne amidite, hydroxyprolinol
Acetylenic amidite for the incorporation of acetylene groups into oligos.FAM phosphoramidite, 5-isomer
Single isomer 5-FAM phosphoramidite for the synthesis of modified oligonucleotides.THPTA ligand
THPTA is a water-soluble ligand for Cu(I)-catalyzed click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.General properties
Appearance: | black powder |
Molecular weight: | 1413.55 |
Molecular formula: | C75H89N12O14P |
Quality control: | NMR 1H, 31P,HPLC-MS (95%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 552 |
Oligo synthesis details
Coupling conditions: | 6 min coupling time |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases |
