DusQ 2 dT phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1451-100mg | 100 mg | $340 | in stock | |
| 1451-500mg | 500 mg |
$1390
|
in stock | |
| 1451-1g | 1 g |
$2290
|
in stock |
DusQ 2 dT phosphoramidite is a modified oligo synthesis reagent bearing completely non-fluorescent quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 2 has large extinction coefficient, efficiently quench fluorescence for dyes emitting in the far red region, such as Cyanine5.
DusQ 2 is typically used to construct qPCR probes with a quencher moiety. Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 2 has a absorption spectrum in of red and near-infrared range and can be paired with reporter dyes that emit in the range 560–670 nm, e.g. TAMRA, ROX, Cyanine3, and Cyanine5.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
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| Appearance: | black powder |
| Molecular weight: | 1413.55 |
| Molecular formula: | C75H89N12O14P |
| Quality control: | NMR 1H, 31P,HPLC-MS (95%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
Oligo synthesis details
| Coupling conditions: | 6 min coupling time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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