ROX azide, 5-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A1230 | 1 mg | $110 | in stock | |
B1230 | 5 mg | $165 | in stock | |
C1230 | 10 mg | $210 | in stock | |
D1230 | 25 mg | $285 | in stock | |
E1230 | 50 mg | $495 | in stock |
ROX azide is an alkyne-reactive derivative of ROX (Rhodamine X, Rhodamine 101) dye. ROX is a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This reagent is a pure 5-isomer. It is used for labeling alkyne and cycloalkyne-containing biomolecules via copper-catalyzed and copper-free click chemistry reactions.
Absorption and emission spectra of ROX dye
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Ascorbic acid
Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.General properties
Appearance: | dark crimson powder / red solution |
Molecular weight: | 616.71 |
CAS number: | 2628213-67-0 |
Molecular formula: | C36H36N6O4 |
Solubility: | good in polar organic solvents (DMF, DMSO, alcohols), low in water |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 570 |
ε, L⋅mol−1⋅cm−1: | 93000 |
Emission maximum, nm: | 591 |
Fluorescence quantum yield: | 1.0 |
CF260: | 0.62 |
CF280: | 0.49 |
Product citations
- Ferguson, T.E.G.; Reihill, J.A.; Martin, S.L.; Walker, B. Novel Inhibitors and Activity-Based Probes Targeting Trypsin-Like Serine Proteases. Frontiers in Chemistry, 2022, 10, 782608. doi: 10.3389/fchem.2022.782608
- Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
- Verardi, R.; Kim, J.-S.; Ghirlando, R.; Banerjee, A. Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase. Structure, 2017, 25(9), 1337–1347. doi: 10.1016/j.str.2017.06.018
- Bak, D.W.; Pizzagalli, M.D.; Weerapana, E. Identifying Functional Cysteine Residues in the Mitochondria. ACS Chemical Biology, 2017, 12(4), 947–957. doi: 10.1021/acschembio.6b01074
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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