ROX azide, 5-isomer

Azides ROX (Rhodamine X, Rhodamine 101) Click chemistry
Cat. # Quantity Price Lead time
A1230 1 mg
$110
 in stock
B1230 5 mg
$165
 in stock
C1230 10 mg
$210
 in stock
D1230 25 mg
$299
 in stock
E1230 50 mg
$495
 in stock
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ROX azide is an alkyne-reactive derivative of ROX (Rhodamine X, Rhodamine 101) dye. ROX is a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This reagent is a pure 5-isomer. It is used for labeling alkyne and cycloalkyne-containing biomolecules via copper-catalyzed and copper-free click chemistry reactions.

ROX azide, 5- isomer, D1230
ROX azide, 5- isomer, D1230
ROX azide, 5- isomer, x1230
Structure of ROX azide, 5-isomer
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Absorption and emission spectra of ROX dye

Absorption and emission spectra of ROX dye

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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

General properties

Appearance: dark crimson powder
Molecular weight: 616.71
CAS number: 2628213-67-0
Molecular formula: C36H36N6O4
Solubility: good in polar organic solvents (DMF, DMSO, alcohols), low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.0
CF260: 0.62
CF280: 0.49

Product citations

  1. Ferguson, T.E.G.; Reihill, J.A.; Martin, S.L.; Walker, B. Novel Inhibitors and Activity-Based Probes Targeting Trypsin-Like Serine Proteases. Frontiers in Chemistry, 2022, 10, 782608. doi: 10.3389/fchem.2022.782608
  2. Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
  3. Verardi, R.; Kim, J.-S.; Ghirlando, R.; Banerjee, A. Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase. Structure, 2017, 25(9), 1337–1347. doi: 10.1016/j.str.2017.06.018
  4. Bak, D.W.; Pizzagalli, M.D.; Weerapana, E. Identifying Functional Cysteine Residues in the Mitochondria. ACS Chemical Biology, 2017, 12(4), 947–957. doi: 10.1021/acschembio.6b01074
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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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