AF 384 (PEP) azide

Azides PEP (Phenylethynyl-pyrene) Click chemistry
Cat. # Quantity Price Lead time
A4530 1 mg
$110
 in stock
B4530 5 mg
$210
 in stock
C4530 10 mg
$310
 in stock
D4530 25 mg
$410
 in stock
E4530 50 mg
$695
 in stock
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Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, AF 384 (PEP) fluorescence is more red-shifted

PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.

This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and click chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.

AF 384 (PEP) azide, D4530
AF 384 (PEP) azide, D4530
Phenylethynylpyrene AF 384 (PEP) azide structure
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Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

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General properties

Appearance: off white / yellowish solid
Molecular weight: 544.64
CAS number: 1807521-02-3
Molecular formula: C34H32N4O3
Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
Quality control: NMR 1H (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 293; 362; 384
ε, L⋅mol−1⋅cm−1: 46300
Emission maximum, nm: 389
Fluorescence quantum yield: 0.64

Product citations

  1. Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/1e
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