AF 343 (Coumarin) X azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12630 | 1 mg | $105 | in stock | |
| 22630 | 5 mg | $165 | in stock | |
| 42630 | 25 mg | $285 | in stock | |
| 52630 | 50 mg |
$495
|
in stock | |
| 62630 | 100 mg |
$880
|
in stock |
AF 343 (Coumarin) is a blue emitting fluorophore with an emission maximum of around 480 nm. This dye forms a FRET pair with fluorescein and can harvest blue light energy for the subsequent transfer to other fluorophores.
The azide derivative can be conjugated with alkynes in copper-catalyzed and copper-free сlick reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye and the azide function.
Absorption and emission spectra of AF 343 (Coumarin)
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Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.General properties
| Appearance: | yellow solid |
| Mass spec M+ increment: | 480.3 |
| Molecular weight: | 480.56 |
| Molecular formula: | C25H32N6O4 |
| IUPAC name: | 5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
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