FAM azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15130 | 100 uL, 10 mM/DMSO | $69 | in stock | |
| 35130 | 500 uL, 10 mM/DMSO | $97 | in stock | |
| 45130 | 1 mL, 10 mM/DMSO | $139 | in stock | |
| A5130 | 1 mg | $69 | in stock | |
| B5130 | 5 mg | $97 | in stock | |
| C5130 | 10 mg | $139 | in stock | |
| D5130 | 25 mg | $285 | in stock | |
| E5130 | 50 mg |
$445
|
in stock | |
| F5130 | 100 mg |
$765
|
in stock |
FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for сlick сhemistry. solid and 10 mM solution in DMSO, labeling reagent for сlick chemistry. This reagent is a pure 6-isomer.
It can replace DyLight 488.
FAM absorbance and emission spectra
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PreciseGreen® is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.FAM azide, 5-isomer
FAM (fluorescein) azide for сlick chemistry labeling, pure 5-isomer.VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.General properties
| Appearance: | yellowish crystals |
| Molecular weight: | 458.42 |
| CAS number: | 1386385-76-7 |
| Molecular formula: | C24H18N4O6 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
Product citations
- Kropacheva, N. O.; Golyshkin, A. A.; Vorobyeva, M. A.; Meschaninova, M. I. Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond. Molecules, 2023, 28(4), 1904. doi: 10.3390/molecules28041904
- Gabold, B.; Adams, F.; Brameyer, S.; Jung, K.; Ried, C. L.; Merdan, T.; Merkel, O. M. Transferrin-Modified Chitosan Nanoparticles for Targeted Nose-to-Brain Delivery of Proteins. Drug Deliv. and Transl. Res., 2023, 13(3), 822–838. doi: 10.1007/s13346-022-01245-z
- Kafle, C. M.; Anderson, A. Y.; Prakash, A.; Swedik, S.; Bridge, E. An Adenovirus Early Region 4 Deletion Mutant Induces G2/M Arrest via ATM Activation and Reduces Expression of the Mitotic Marker Phosphorylated (Ser10) Histone 3. Virology, 2022, 565, 1–12. doi: 10.1016/j.virol.2021.09.006
- Babi, M.; Palermo, A.; Abitbol, T.; Fatona, A.; Jarvis, V.; Nayak, A.; Cranston, E.; Moran-Mirabal, J. Direct Imaging of the Alternating Disordered and Crystalline Structure of Cellulose Fibrils via Super-Resolution Fluorescence Microscopy. ChemRxiv, 2022, preprint. doi: 10.26434/chemrxiv-2022-0jqng
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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