FAM hydrazide, 6-isomer

Cat. # Quantity Price Lead time
15170 1 mg $110 in stock
25170 5 mg $210 in stock
45170 25 mg $410 in stock
55170 50 mg $695 in stock
65170 100 mg $1190 in stock
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Fluorescein (FAM) is a xanthene dye, a parent of fluorescein dye series. This is a hydrazide derivative for the labeling of carbonyl compounds, aldehydes, and ketones.

Fluorescein is a bright dye that is compatible with a great number of instruments.

Carbohydrates are also often labeled with hydrazides by a protocol involving their oxidation with periodate first, and reacting with dye hydrazide after it.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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Alkyne hydrazide

Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.

General properties

Appearance: yellow/orange solid
Mass spec M+ increment: 371.1
Molecular weight: 426.81
CAS number: 2183440-65-3 (hydrochloride); 151890-73-2
Molecular formula: C21H15N2ClO6
IUPAC name: Spiro[isobenzofuran-​1(3H)​,​9'-​[9H]​xanthene]​-​6-​carboxylic acid, 3',​6'-​dihydroxy-​3-​oxo-​, hydrazide, hydrochloride
Solubility: good in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 522
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17
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