HEX azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| AB230 | 1 mg | $110 | in stock | |
| BB230 | 5 mg | $165 | in stock | |
| CB230 | 10 mg | $210 | in stock | |
| DB230 | 25 mg | $285 | in stock | |
| EB230 | 50 mg |
$495
|
in stock | |
| FB230 | 100 mg |
$880
|
in stock |
Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.
Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition.
HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in green-yellow area, and it can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.
Absorption and emission spectra of HEX
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TR-X-carboxylic acid, 5-isomer
Carboxyl derivative of TR, a red-emitting fluorescent dye. It can be used as a reference standard or for synthesizing various TR esters. The extended aminohexanoyl linker increases solubility and provides spatial separation between the fluorophore and the functional group.
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VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 661.9 |
| Molecular weight: | 665.09 |
| CAS number: | 1450752-91-6 |
| Molecular formula: | C24H12N4Cl6O6 |
| IUPAC name: | N-(3-azidopropyl)-2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamide |
| Solubility: | very soluble in DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 87770 |
| Emission maximum, nm: | 549 |
| Fluorescence quantum yield: | 0.57 |
| CF260: | 0.30 |
| CF280: | 0.13 |
BODIPY™, VIC™ are trademarks of Thermo Fisher Scientific.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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