HEX phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| CB260 | 100 mg | – | 5 days | |
| DB260 | 250 mg | $272 | in stock | |
| FB260 | 1 g |
$575
|
in stock | |
| GB260 | 5 g |
$2640
|
in stock | |
| HB260 | 10 g | please inquire | in stock |
HEX phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
HEX (hexachlorofluorescein) is a fluorescein derivative with emission in the yellow spectrum range (absorption maximum at 533 nm, emission maximum at 549 nm).
HEX phosphoramidite is used for synthesis of fluorescent-labeled primers and hybridization probes such as TaqMan, Molecular Beacon, and Scorpion for qPCR. HEX is most effectively quenched by non-fluorescent DusQ 1 dark quencher because of significant overlapping of their spectra (convenient for use with DusQ 1 CPG 500 solid support with a pore size of 500 Å). Many automated sequencers based on capillary gel electrophoresis have a detection channel for HEX. Therefore, this phosphoramidite is commonly used for synthesis of 5’-labeled oligonucleotides for fragment analysis, particularly for microsatellite analysis, when microsatellite loci are amplified using a fluorescent-labeled forward primer and a non-labeled reverse primer.
Usage
Coupling: 3 min.
Deprotection: standard conditions using 25% ammonium; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of concentrated aqueous ammonium/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
Absorption and emission spectra of HEX
Customers also purchased with this product
FAM-dT phosphoramidite
Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.
Add this product to your cart andget free express delivery
ATT 550 azide
Azide derivative of water-soluble yellow-fluorescent dye ATT 550 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.DusQ 2 CPG 500
Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
Add this product to your cart andget free express delivery
General properties
| Appearance: | off white solid |
| Molecular weight: | 1050.61 |
| CAS number: | 1360547-55-2 |
| Molecular formula: | C46H52N3Cl6O10P |
| Solubility: | Good solubility in acetonitrile and DCM. |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 87770 |
| Emission maximum, nm: | 549 |
| Fluorescence quantum yield: | 0.57 |
| CF260: | 0.30 |
| CF280: | 0.13 |
Oligo synthesis details
| Diluent: | anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week). |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
Your cart is empty
$ 
