HMRhoNox-M, Fe(II)-selective fluorescent probe

Microscopy Flow cytometry

Cat. #	Quantity	Price	Lead time	Buy this product
3317-50ug	50 ug	$120	in stock
3317-500ug	500 ug	$590	in stock

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HMRhoNox-M (also known as LysoRhoNox) is a Fe²⁺-selective fluorescent probe based on the N-oxide-controlled spirocyclization of tetramethyl-hydroxymethyl rhodamine.

In the absence of Fe²⁺, HMRhoNox-M exists in the non-fluorescent spirocyclic form showing only negligible fluorescence in an aqueous buffer and at physiological pH. The addition of Fe²⁺ induces a 60-fold increase of the fluorescence signal at 575 nm through the deoxygenation of the dialkylamino group and the transition of the probe to an open fluorescent form. HMRhoNox-M responds to Fe²⁺ in a dose-dependent manner.

The fluorescence response of HMRhoNox-M is highly selective for Fe²⁺ over other transition metal ions, including Fe³⁺, alkali metal ions, and alkaline earth metal ions.

HMRhoNox-M is the cell-permeant probe that is mainly localized in lysosomes. It is suitable for monitoring fluctuations of endogenous labile iron in living cells, including the transferrin-induced Fe uptake.

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A549 cells stained with Fe(II)-selective fluorescent probe HMRhoNox-M (3317-x) and DAPI

Absorption and emission spectra of HMRhoNox-M

General properties

Appearance:	beige-pinkish crystals
Molecular weight:	388.47
Molecular formula:	C₂₄H₂₄N₂O₃
Solubility:	DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	555
Emission maximum, nm:	575

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.