LumiTracker® Mito TMRE
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2274-1mg | 1 mg | – | in stock | |
| 2274-25mg | 25 mg | $190 | in stock |
TMRE is widely used for labeling mitochondria in live cells but not compatible with fixation. This lipophilic and positively charged dye rapidly permeates plasma membrane without interacting with membrane proteins and forming aggregates. TMRE selectively accumulates in active mitochondria due to their transmembrane potential.
In addition to staining mitochondria for imaging purposes, TMRE is used for quantitative measurements of mitochondria membrane potential using Nernst equation. The dye serves as a tool to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest. Mitochondrial depolarization caused by apoptosis, necrosis or other factors is characterized by decreased membrane potential and is indicated with decreased fluorescence compared to intact cells that have polarized mitochondria.
TMRE applications include fluorescent microscopy, flow cytometry, microplate assays. The dye has an excitation maximum at 549 nm: it can be effectively excited by the blue (488 nm) or yellow-green (561 nm) lasers. Emission of the dye can be detected in PE channel (maximum at 574 nm).
Absorption and emission spectra of TMRE
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| Appearance: | dark colored solid |
| Molecular weight: | 514.96 |
| CAS number: | 115532-52-0 |
| Molecular formula: | C26H27N2ClO7 |
| IUPAC name: | 3,6-bis(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthylium perchlorate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 156000 |
| Emission maximum, nm: | 575 |
| Fluorescence quantum yield: | 0.66 |
Product citations
- Mikheeva, A.M.; Bogomolov, M.A.; Gasca, V.A.; Sementsov, M.V.; Spirin, P.V.; Prassolov, V.S.; Lebedev, T.D. Improving the power of drug toxicity measurements by quantitative nuclei imaging. Cell Death Discovery, 2024, 10, 181. doi: 10.1038/s41420-024-01950-3
- Moiseeva, N.; Eroshenko, D.; Laletina, L.; Rybalkina, E.; Susova, O.; Karamysheva, A.; Tolmacheva, I.; Nazarov, M.; Grishko, V. The Molecular Mechanisms of Oleanane Aldehyde-β-Enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells. Biology, 2023, 12(3), 415. doi: 10.3390/biology12030415
- Kozlova, T.; Popov, A.; Romanov, M. V.; Savintseva, I.; Vasilyeva, D. N.; Baranchikov, A.; Ivanov, V. Ceric Phosphates and Nanocrystalline Ceria: Selective Toxicity to Melanoma Cells. Nanosystems: Physics, Chemistry, Mathematics, 2023, 14, 223–230. doi: 10.17586/2220-8054-2023-14-2-223-230
- Tolmacheva, I.; Beloglazova, Y.; Nazarov, M.; Gagarskikh, O.; Grishko, V. Synthesis and Anticancer Activity of A-Ring-Modified Derivatives of Dihydrobetulin. International Journal of Molecular Sciences, 2023, 24(12), 9863. doi: 10.3390/ijms24129863
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